No. 1 contained an ointment made with lard.
No. 2, one made with unguentum paraffini (_Germ. Pharm_.), and
No. 3, one made with unguentum paraffini mixed with 3 per cent. of lard.
All three vials were then suspended in beakers filled with water. After
standing twenty-four hours at the ordinary temperature, the contents of
none of the beakers gave any iodine reaction. After having been placed
into a warm temperature, between 25-37 deg. C., all three showed iodine
reactions after three hours, Nos. 2 and 3 very strongly, No. 1 (with
lard alone) very faintly.
The same experiment was now repeated, with the precaution that the
bladder was previously washed completely free from chlorine. Each vial
was suspended, at a temperature of 25-27 deg. C., in 50 grammes of distilled
water. After three hours, the contents of No. 1 (containing the ointment
made with _lard_) gave _no_ iodine reaction; the contents of the other
two, however, gave traces. After eight hours no further change had taken
place. The temperature was now raised to 30-35 deg. C., and kept so for
eight hours. All three beakers now gave a strong iodine reaction, 0.2
c.c. of normal silver solution being required for each 15 grammes of the
contents of the beakers.
In addition to the iodide, some of the fatty base had osmosed through
the membrane in each case.
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